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KMID : 0352819930090020023
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Kosin Medical Journal
1993 Volume.9 No. 2 p.23 ~ p.38
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The effect of kojic acid and pentadecenoic acid on the invitro and in vivo pigmentary system after Ultraviolet-B (UVB) irradiation
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Abstract
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It has been well known that exposure to ultraviolet-B(UVB) light elicits increased pigmentation in the skin. The model has been widely adapted to assess the potential of chemicals as compounds with skin-depigmenting effectsl. We observed the
pigment
inhibitory effect of kojic acid(KA) and pentadecenoic acid(PDA) on the cultured human melanocytes and C57BL mice skin after UVB irradiation.
Cultured human melanocytes were irradiated with 30mJ/cm2 of of UVB cnce, than KA and PDA were adminstered for 3 or 5 days. C57BL black mice were irradiated with 100mJ/cm2 of UVB daily for 10 days, and then KA and PDA were topically applied daily
for 1,
3, 5 or 7 weeks. For demonstration of the effect ofboth drugs, we observed numeric and morphologic changes and measured melanincontents of cultured normal human melanocytes Also we examined the effect of the chemicals on split-DOPA stained
epidermal
melanocytes of C57BL mice.
@ES The resulis were as follows:
@EN 1. After UVB-irradiation, cell number and melanin content decreased initially, but melanin content increased after 5 days incultured human melanocytes.
2. In cultured human melanocytes, the KA 10-3M groupshowed decreased number of melanocyte, but KA 10-5M or PDA groups showed no change.In all experimertal groups, melanin content decreased, compared to control group.
3. After UVB-irradiation, the number ofculture human melanocytes decreased in groups treated with KA 10-3M for 3 and 5 days, KA 10-5M for 3 days and PDA 10-3M for 5 days compared to UVB control group.
4. In C57BL mice, the KA group showed decreased number of melanocytes compared to control group. The PDA group showed no change. After UVB-irradiation, both KA and PDA groups showed decreased number of melanocytes compared to UVB control group.
In the present study, it was found that KA and PDA had suppressive effects on melanization of melanocytes in vitro and in vivo, suggesting KA and PDA might be candidates as compounds that control hyperpigmentary disoredrs.
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KEYWORD
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